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KMID : 0381920090390010027
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Korean Journal of Microscopy
2009 Volume.39 No. 1 p.27 ~ p.40
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The Change of Alveolar-capillary Barrier by Germanium in Acute Lung Injury Induced by Lipopolysaccharide
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Lee Yoon-Jeong
Chun Kyung-Hee
Cho Hyun-Gug
Sin Gun-Ho
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Abstract
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Acute respiratory distress syndrome (ARDS), also known as an acute inflammatory lung disease is developed by various factors that is originated from the destruction of alveolar-capillary barrier, and neutrophils plays an important role in the destruction. The study intended to confirm, the anti-inflammatory effect of germanium, whether a lung injury has been mitigated with the reduction of injury in alveolar-capillary barrier resulting from inhibition of neutrophils migration in lung tissue. Test groups were divided in saline administered CON, 5 hours of endotoxin administered LPS and 5 hours of endotoxin administered Ge+LPS following 1 hours of pre-processed germanium. 100 μg endotoxin was melted in 0.5mL saline and sprayed into airway and 26 mg germanium per 100 g weight was administered into abdominal cavity. The endotoxin group which induced an acute lung injury with administered endotoxin showed dramatic increase of pulmonary edema (p?0.001), protein contents in bronchoalveolar lavage fluid, BALF (p?0.05) and neutrophils of infiltration in BALF (p?0.001) comparing with a control group, while a pre-treated germanium group showed significant decrease in all categories comparing to the endotoxin administerd group. In the result of a microscopic observation, the structure of alveolar-capillary barrier which is constructed with basal lamina, alveolar type I cells and endothelial cel
were preserved of the pre-treated germanium group relatively well compare to the endotoxin administered group. And the construction of lamellar body, microvilli and basal lamina of alveolar type II cells were also preserved relatively well.
Hence, germanium activates as an anti-Inflammatory mediator in other words, it interfered neutrophils migration into lung tissue, thereby reduced injury of alveolar-capillary barrier from toxic substances of activated neutrophils. Consequently, the study has determined that the acute lung injury induced by endotoxin has been decreased by the pre-treated ermanium.
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KEYWORD
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Acute lung injury, Germanium, Alveolar-capillary barrier
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